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American Society of Microbiology ABMM Sample Questions:

1. A patient undergoing chemotherapy for leukemia develops a severe pneumonia. Bronchoalveolar lavage fluid is examined, revealing numerous cysts containing crescent-shaped structures upon silver staining. The patient's CD4+ T-cell count is significantly low. The MOST likely diagnosis is infection with:

A) Pneumocystis jirovecii
B) Blastomyces dermatitidis
C) Coccidioides immitis
D) Histoplasma capsulatum

2. In a clinical microbiology laboratory, quality control testing of Mueller-Hinton agar used for disk diffusion susceptibility testing involves checking the zone sizes for E. coli ATCC 25922, S. aureus ATCC 25923, and
P. aeruginosa ATCC 27853. What is the primary purpose of including P. aeruginosa ATCC 27853 in this QC?

A) To verify the performance of cell wall active agents like penicillin
B) To ensure appropriate concentration of calcium and magnesium ions affecting aminoglycoside activity
C) To confirm the correct pH of the agar medium
D) To assess the performance of agents primarily active against Gram-positive organisms

3. A microbiology laboratory is investigating an outbreak of bloodstream infections in a neonatal intensive care unit. Isolates of Staphylococcus epidermidis from multiple infants show indistinguishable banding patterns by pulsed-field gel electrophoresis (PFGE). This finding MOST strongly suggests:

A) Recent horizontal gene transfer of virulence factors.
B) Spontaneous mutations leading to increased pathogenicity.
C) A common source of infection and clonal spread of the organism.
D) Widespread antibiotic resistance within the S. epidermidis population.

4. A researcher is studying the genetic relatedness of different isolates of Mycobacterium tuberculosis. Which of the following molecular typing methods provides the highest discriminatory power for distinguishing between closely related strains?

A) Whole-genome sequencing (WGS)
B) Spoligotyping
C) Pulsed-field gel electrophoresis (PFGE)
D) Restriction fragment length polymorphism (RFLP) analysis

5. A clinical microbiology laboratory is performing quality control on antimicrobial susceptibility testing. The zone diameter for Escherichia coli ATCC 25922 tested against ciprofloxacin is consistently smaller than the acceptable range published by CLSI. The MOST likely cause of this discrepancy is:

A) Incubation at an incorrect temperature
B) Use of expired antibiotic disks
C) Inoculation with a bacterial suspension that is too dense
D) All of the above

Solutions: